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large clone light microscope

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large clone SEM

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small clone light microscope

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small clone SEM

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Chromosome: 3L

Genotype: y[d2] w[1118] P{ry[+t7.2]=ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1;y[d2] w[1118] P{ry[+t7.2]=ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1;P{w[+mC]=lacW}RpS12[s2783] P{ry[+t7.2]=neoFRT}80B/TM6B, P{y[+t7.7] ry[+t7.2]=Car20y}TPN1, Tb[1]P{ry[+t7.2]=neoFRT}80B/TM6B, P{y[+t7.7] ry[+t7.2]=Car20y}TPN1, Tb[1]

Link to parent stock

Insertion Site: 069F05

gene disrupted 1: Ribosomal protein S12

Genomic Insertion

Large Clone Phenotype Keyword(s): cell lethal

Phenotype Description: Large clones are observed, but they are not true mosaics since the orange clones are absent due to the cell-lethal phenotype. The light microscope picture shows the presence of only red clones in the eye. The SEM picture shows disorganized ommatidia and that some of the ommatidia have fused together in the cell-lethal mutants. Also, some of the ommatidia formed different shapes than the regular hexagon shape and other have holes. Additionally, a small patch shows loss of structure. The orange/white mosaic eyes are observed for small clone analysis. These phenotypes have greatly expanded white clones, which supports the cell-lethal large clone observation. The small clone SEM picture displays a rough phenotype.

Reversion to wild type eye by excision: Yes

This stock is available here: http://kyotofly.kit.jp/stocks/dgrccart.php?selected_no=111390