| large clone light microscope | large clone SEM | small clone light microscope | small clone SEM |
|
Chromosome: 3L Genotype: y[d2] w[1118] P{ry[+t7.2]=ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1;y[d2] w[1118] P{ry[+t7.2]=ey-FLP.N}2P{GMR-lacZ.C(38.1)}TPN1; P{w[+mC]=lacW}Neos[L2249] P{ry[+t7.2]=neoFRT}80B/TM6B, P{y[+t7.7]ry[+t7.2]=Car20y}TPN1, Tb[1]P{ry[+t7.2]=neoFRT}80B/TM6B, P{y[+t7.7] ry[+t7.2]=Car20y}TPN1, Tb[1] Insertion Site: 065E09 gene disrupted 1: Neosingene disrupted 2: Ribosomal protein L18 Large Clone Phenotype Keyword(s): cell lethal Phenotype Description: Large clone analysis shows the cell lethal phenotype (red and dark red in color). The light microscope pictures show large shiny areas as well as a noticeable reduction in the size of the eye. SEM photo shows fusion of ommatidia, partial loss of ommatidia structure, and unusual bristle pattern (randomness in smaller ommatidia areas and none in fused ommatidia area). Orange/white mosaic eyes are observed in the small clone analysis. The homozygous mutant twin spot clones are evident (darker and lighter orange) but difficult to visualize, yet the SEM picture shows the orange tissues to be slightly rough. Reversion to wild type eye by excision: Yes This stock is available here: http://kyotofly.kit.jp/stocks/dgrccart.php?selected_no=111003 |
|||