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large clone light microscope

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large clone SEM

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small clone light microscope

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small clone SEM

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Chromosome: 3L

Genotype: y[d2] w[1118] P{ry[+t7.2]=ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1;y[d2] w[1118] P{ry[+t7.2]=ey-FLP.N}2P{GMR-lacZ.C(38.1)}TPN1; P{w[+mC]=lacW}Neos[L2249] P{ry[+t7.2]=neoFRT}80B/TM6B, P{y[+t7.7]ry[+t7.2]=Car20y}TPN1, Tb[1]P{ry[+t7.2]=neoFRT}80B/TM6B, P{y[+t7.7] ry[+t7.2]=Car20y}TPN1, Tb[1]

Link to parent stock

Insertion Site: 065E09

gene disrupted 1: Neosin

gene disrupted 2: Ribosomal protein L18

Genomic Insertion

Large Clone Phenotype Keyword(s): cell lethal

Phenotype Description: Large clone analysis shows the cell lethal phenotype (red and dark red in color). The light microscope pictures show large shiny areas as well as a noticeable reduction in the size of the eye. SEM photo shows fusion of ommatidia, partial loss of ommatidia structure, and unusual bristle pattern (randomness in smaller ommatidia areas and none in fused ommatidia area). Orange/white mosaic eyes are observed in the small clone analysis. The homozygous mutant twin spot clones are evident (darker and lighter orange) but difficult to visualize, yet the SEM picture shows the orange tissues to be slightly rough.

Reversion to wild type eye by excision: Yes

This stock is available here: http://kyotofly.kit.jp/stocks/dgrccart.php?selected_no=111003