705 UCLA Undergraduate Research Consortium in Functional Genomics -- sponsored by the Howard Hughes Medical Institute -- UCLA Undergraduate Research Consortium in Functional Genomics -- bloomington 3L (FRT 80B) - stock#: 12095
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Home    1. Authors    2. Database    3. Methods Figure S1 - Crossing Scheme Figure S2 - FLP/FRT Verification and Excision Experiments Figure S3 - Excision Scheme BLAST Hit Determination    4. Education The Class Didactic Component Computer Component Laboratory Component Sample Syllabus Sample Midterm Sample Final    5. Manuscript    6. Class Website    7. Advanced
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large clone light microscope

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large clone SEM

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small clone light microscope

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Chromosome: 3L

Genotype: y[d2] w[1118] P{ry[+t7.2]=ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1;y[d2]w[1118] P{ry[+t7.2]=ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{w[+mC]=lacW}l(3)72Dn[j5A4]P{ry[+t7.2]=neoFRT}80B/TM6B, P{y[+t7.7] ry[+t7.2]=Car20y}TPN1, Tb[1]P{ry[+t7.2]=neoFRT}80B/TM6B, P{y[+t7.7] ry[+t7.2]=Car20y}TPN1, Tb[1]

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Insertion Site: 072D10

gene disrupted 1: CG5018

gene disrupted 2: CG5027

Genomic Insertion

Large Clone Phenotype Keyword(s): cell lethal

Phenotype Description: All red eyes are observed for large clone analysis. The light microscope picture shows that the eyes are mostly red, and that they display cell lethal phenotypes. The SEM picture shows the disorganized ommatidia and that some ommatidia are underdeveloped. These underdeveloped ommatidia also lack pigmentation. Also, orange/white mosaic eyes are observed for small clone analysis. The homozygous twin spots are difficult to visualize. (There is no dark orange versus orange.) The eyes are wild-type mosaic.

Reversion to wild type eye by excision: Yes

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